Acidum nuclei extractionis Kit (A01)
Deprehensio principium
Postquam DNA genomica solvens cellulas cum lysis quiddam scindendo, magnetica grana selective ligare potuerunt ad DNA genomicum in sample.Parvus numerus immunditiae quae a globulis magneticis absorpta est, quiddam lavacrum tolli potuit.In TE, globulum magneticum DNA limitem emittere potuit, obtinens genome DNA GENERALE.Haec methodus simplex est et velox et extracta qualitatis DNA alta est, quae postulationem occurrere potuit pro methylatione detectionis DNA.Interim ornamentum extractionis secundum magneticam capita compatible cum automatic acidum nuclei extraction, occurrens princeps throughput nuclei operis extractionis acidi.
Principalis components de tenui
Partes ostenduntur in tabula 1;
Table I Reagent Components et Loading
| Nomen component | Principalis components | Magnitudo (48) | Size (200) |
| 1. Lysis solutionem | Guanidinum hydrochloridum, Tris | 11 mL/utrem | 44 mL/utrem |
| 2. Purgatio solutiones A | NaCl, Tris | 11 mL/utrem | 44 mL/utrem |
| 3. Purgatio solutiones B | NaCl, Tris | 13 mL/utrem | 26.5mL/fel *2 |
| 4. Eluent | Tris, EDTA | 12 mL/utrem | 44 mL/utrem |
| 5. K solution | Protease K | 1.1mL/piece | 4.4mL/piece |
| 6. Magnetica capita suspensionis 1 | Grana magnetica | 1.1mL/piece | 4.4mL/piece |
| 7. Instructiones ad reagentes acidi nuclei extrahendos |
| 1 copy | 1 copy |
Components quae requiruntur in extractione acidi nuclei, sed in ornamento non continentur;
1. Reagent: Anhydrous ethanol, isopropanol et PBS;
2. Consumables: 50ml tubum centrifugium and 1.5ml EP tube;
3. Apparatus: Aquae balnei, ollae, pipettoris, pluteae magneticae, centrifugium, fovea 96-bracteae profundae (automaticae), instrumenti extractionis acidi nucleici automatici (automatici).
Basic notitia
Sample requisita
1. Crux contaminatio inter exempla vitanda est in collectione sample et repositione.
2. Deprehensio sub 7-die ceptaculo temperie ambientis complebitur post collectionem speciminis cellulae exfoliatae ceruicis (non-fixa).Deprehensio sub 30-die ceptaculo temperatura ambientis complebitur post collectionem speciminis urinae;Deprehensio tempore complebitur post collectionem speciminum cellularum excultarum.
Raeda specificatio:200 pcs/arca, 48 pcs/box.
Repono conditiones:2-30℃
Periodus validitatis:XII mensibus
Lorem fabrica:Tianlong NP968-C Instrumentum extractionis acidi nucleici, Tiangen TGuide S96 Instrumentum extractionis acidi nucleici, GENE DIAN EB-1000 Instrumentum extractionis acidi nucleici.
Medical fabrica record libellum No./product postulationem technicam No.: HJXB No.
Date approbationem et modificationem mandatorum:
Approbatio diei 18 Nov
